Expression of Malignancy in Somatic Cell Hybrids
نویسندگان
چکیده
منابع مشابه
Expression of tumor-specific antigens in mouse somatic cell hybrids.
Inbred 129/Sv mice were resistant to inoculation of teratocarcinoma cells when preimmunized with (129teratocarcinoma x C3H L-cell) somatic cell hybrids ob tained by fusion with UV-inactivated Sendai virus. Re sistance of DBA/2 mice to the highly malignant L1210 leukemia was also obtained by the use of (L1210 x C3H L-cell) hybrids. That this resistance was specific was sug gested by regular take...
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The techniques described permit the controlled production of large numbers of proliferating somatic cell hybrids in a relatively short period of time. Sendai virus is used to promote cell hybridization. beta-propriolactone is employed as the inactivating agent of Sendai virus since it produces complete loss of viral infectivity while preserving viral fusion capacity. Cells are fused in monolaye...
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Monoclonal antibodies to carcinoembryonic antigen (CEA) promise improved specificity for the measurement of this widely expressed human cancer antigen. A mouse monoclonal antibody binds weakly to CEA in perchloric acid extracts of tumour but strongly to CEA similarly isolated from serum, and its spectrum of cancer detection differs from conventional antisera.
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Hybrid cell lines, derived from fusion of rodent cells with peripheral leukocytes from patients with chronic myeloid leukaemia (CML) and from normal donors, were assayed for the present of CML-associated changes in the carbohydrate moieties of membrane glycoproteins. Expression of this characteristic phenotype did occur in several hybrid clones derived from fusions both with leukaemic and norma...
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In order to study whether cell fusion would modify the DNA copy number of an amplified oncogene, somatic cell hybrids were made between the human neuroepithelioma cell line MCIXC and HeLaCOT human adenocarcinoma cells. MCIXC contains approximately 21 copies of the c-myc oncogene and HeLaCOT contains approximately 5 copies relative to the control. All hybrid clones investigated displayed a marke...
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ژورنال
عنوان ژورنال: Nature
سال: 1970
ISSN: 0028-0836,1476-4687
DOI: 10.1038/227067a0